DAYA HAMBAT AIR PERASAN JERUK NIPIS (Citrus aurantifolia S.) PADA PERTUMBUHAN BAKTERI Edwardsiella tarda DARI BENIH LELE DUMBO (Clarias gariepinus) SECARA IN VITRO
Abstract
Tujuan dari penelitian ini adalah untuk mengetahui seberapa besar daya hambat air perasan buah jeruk nipis (Citrus aurantifolia S.) terhadap pertumbuhan bakteri Edwardsiella tarda yang diambil dari benih lele dumbo (Clarias gariepinus) secara in vitro. Metode penelitian yang digunakan adalah eksperimental dengan rancangan percobaan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 4 ulangan, 6 perlakuan dan 2 kontrol. Perlakuan yang digunakan adalah pemberian konsentrasi air perasan buah jeruk nipis yang berbeda yaitu Perlakuan A (15%), B (30%), C (45%), D (60%), E (75%), dan F (90%), kemudian kontrol positif (Chloramphenicol) dan kontrol negatif (Aquades steril). Hasil penelitian menunjukkan rata-rata diameter zona hambat tertinggi terdapat pada perlakuan F (dengan nilai rata-rata 17,25 mm) dan rata-rata diameter zona hambat terendah yaitu perlakuan A (dengan nilai rata-rata 1,125 mm). Hubungan antara pemberian air perasan buah jeruk nipis dalam menghambat pertumbuhan bakteri Edwardsiella tarda dengan rata-rata diameter zona hambat yang dihasilkan menunjukkan respon yang meningkat seiring dengan bertambahnya dosis. Berdasarkan uji Anova dan BNT menunjukkan bahwa air perasan buah jeruk nipis (Citrus aurantifolia S.) pada masing-masing perlakuan berpengaruh nyata terhadap daya hambat pertumbuhan bakteri Edwardsiella tarda dengan F hit > F tabel pada taraf signifikan 5 % (2.77) maupun 1% (4.25).
Kata Kunci: Air perasan buah jeruk nipis, Edwardsiella tarda, Uji Daya Hambat
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